1D Analysis

Sitting at the core of ImageQuant TL is a module for fully automatic analysis of 1D gels. It is perfect for a range of functions, from molecular size determination to accurately quantitating band material.

Automatic Analysis

• Single click auto-analysis even for multi-channel files
  
• Includes analysis of multi-tier gels
  
• Refine any step of the analysis
  

Manual options for added flexibility and control

• Proceed through the process step-by-step if desired
  
• Quick Help pane, visible in each mode, to guide new users
  

Lane Creation

• Fully automatic, semi-automatic or manual options
  
• Grimace tool to account for distorted gels
  
• Comprehensive editing tools available
  

Background Subtraction

• Vital for accurate quantitation
  
• Variety of automatic methods to suit different applications
  
• Instantly check results in lane profile window
  

Band Detection

• Fully automatic
  
• Adjustable parameters for optimised band detection
  
• Parameters can be individually adjusted for each channel
  
• Easily add, move or delete bands and edges in the image and on the lane profile
  

Molecular Weight Determination

• Automated, flexible and accurate molecular weight determination
  
• Use supplied standard library, or create your own
  
• Propagate standard from one channel to the other channels
  
• Choice of curve fitting options
  
• Facility to propagate by Rf to account for distorted gels
  

Normalisation

• Overcome the problems associated with experimental variation between lanes and gels
  
• Calculate based on a single band or a group of bands
  

Quantity Calibration

• Accurately calibrate measured quantities to known volumes
  
• Produce a curve from which unknown values are calculated
  
• Account for non-linear staining effects
  

And the results?
There is a range of ways of viewing, editing and reporting on your data, all of which can be copied or exported into other packages.

• Image Window and Profile Window - Edit lanes, bands and edges, zoom in and out, rescale and view in false colour
  
• Measurements Table - a choice of 14 data fields and the option to show all lanes, selected lanes or lane comparisons
  
• Graph Window - for reviewing the Molecular Weight and Quantity Calibration curves
  
• Report Window - produce a customisable report, which takes away the headache of documenting gel data for future reference
  

Evaluate ImageQuant TL - download your copy now.