Automatic spot detection
Analysing and comparing complex 2D electrophoresis samples to a high standard was until now only possible with a great deal of tedious user-input that could take many hours and was, by its very nature, subjective.

Ettan Progenesis automated spot detection does a thorough job with very little user input. After a quick set up you can walk away and rely on the software to apply the same process to every gel, giving you unprecedented confidence in your results.

We can show here a complicated selection of proteins that have been split by Ettan Progenesis.

Splitting of proteins by Ettan Progenesis

Spot detection uses no parameters whatsoever, enabling total objectivity, previously impossible where manual intervention was required. The user does not even need to intervene when faint spots and spot 'shoulders' are present. Ettan Progenesis effortlessly deals with these problems ensuring all material is accurately defined across the gel. Shown below is an example of automatic detection that highlights faint spots and spot shoulders.

Faint spot that has been detected in the shadow of a saturated group

A spot shoulder which was detected by Ettan Progenesis, clearly visible on the 3D view.

Ettan Progenesis is also better at detecting the boundaries of spots, aiding correct background subtraction levels which are calculated on a per spot basis. It may at first look as if the spots have been over defined. When viewed in 3D it is evident that Ettan Progenesis has got it right.

Ettan Progenesis gets spot boundaries right - vital for any form of quantitative comparison.

Noise on an image is a well-documented problem in 2D electrophoresis analysis. Ettan Progenesis has detected these spots and ignored the spikes, which are noise that may have been introduced within the gel itself or during the scanning process.

Please contact us for more information, email: im@imsupport.com

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